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Load example:
BLAST program:
BLASTN - nucleotide query to nucleotide db
BLASTX - translated (6 frames) nucl. query to protein db
BLASTP - protein query to protein db
TBLASTN - protein query to translated (6 frames) nt db
TBLASTX - transl. (6 frames) nucl. query to transl (6) nt db
Query sequence:
Upload File:
Select Blast DB:
+ Settings
Scoring matrix:
BLOSUM62
PAM30
PAM70
BLOSUM80
BLOSUM45
Filtering:
Low complexity
Lower-case letters
E-value cutoff:
1e-3
1e-30
1e-10
1e-5
0.1
0
10
100
1000
Advanced Options:
ungapped
megablast
Query genetic code:
Standard
Vertebrate Mitochondrial
Yeast Mitochondrial
Mold, Protozoan, and Coelocoel Mitochondrial
Invertebrate Mitochondrial
Ciliate Nuclear
Echinoderm Mitochondrial
Euplotid Nuclear
Bacterial
Alternative Yeast Nuclear
Ascidian Mitochondrial
Flatworm Mitochondrial
Blepharisma Macronuclear
DB genetic code:
Standard
Vertebrate Mitochondrial
Yeast Mitochondrial
Mold, Protozoan, and Coelocoel Mitochondrial
Invertebrate Mitochondrial
Ciliate Nuclear
Echinoderm Mitochondrial
Euplotid Nuclear
Bacterial
Alternative Yeast Nuclear
Ascidian Mitochondrial
Flatworm Mitochondrial
Blepharisma Macronuclear
Frame shift penalty:
Word size:
Number of results per sequence:
50
10
100
1
BLAST
Results..
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